Auxiliary measures to improve the survival rate of transplanting In recent years, due to the increasing use of rapid propagation of test-tube seedlings, more and more plant species and varieties are involved, and the climatic conditions and equipment conditions vary widely from place to place. In the process of transplanting test-tube seedlings, many problems have been encountered, and some techniques and measures to improve the survival rate of transplanting have been summarized. MEASURING TAPE,STEEL TAPE MEASURE, NYLON COATED TAPE HENAN BONTHE MEASURING TOOLS. CO., LTD , https://www.btemeasuretool.com
Bu) The rooting of micropropagated plantlets is directed to the reason why the above-mentioned tube seedlings are easily wilted and died under low humidity.
Use the following measures and methods:
1. Promote the recovery and functional recovery of test tube seedlings. Some plant species are difficult to root in test tubes. Or roots but not in contact with the vascular bundle of the stem, or the poor connection between the root and the stem, the absorption function is extremely weak. After transplanting, it is not easy to survive. After the survival, the chlorophyll meter measures its leaf surface, and its chlorophyll content is low, which requires the use of external rooting method.
(1) Miniature cuttings Cutting some plants can use the test tube breeding shoots as micro-cutting, directly into the soil rooting, can also survive, but it is best to use hormone treatment. The cuckoo's twigs are well rooted inside and outside the test tube. Some laboratories have costed and considered that the cost of inducing the rooting process of tube seedlings accounts for 35% of the total cost. However, transplanting with rootless seedlings will kill a lot. At this time, the method of rooting outside the bottle can be used to combine the rooting and domestication processes, which can greatly reduce the cost and increase the survival rate of transplanting.
(2) Rooting and refining in a rooting chamber to make a rooting chamber without agar and sucrose. Use a breathable and moisturizing substrate such as peat, hydrocarbon stone, perlite, moss, etc., and manually control the temperature and light. Using artificial spray, the mist is finely summarized to increase the humidity of the air without causing water to flow out from the foliage. The cut stems of the long 3 stalks are transferred into the rooting chamber, and after 3-4 weeks of cultivation, the well-developed, absorbent roots can be produced. It is then planted in the greenhouse and is very easy to survive. The rooting chamber has a good effect and the conditions are easy to control, but the cost is high and the area is small.
(3) When the test tube seedlings are transplanted after inducing the root primordium in the test tube, if the root is long. It is not good, because the long roots are easy to mechanically damage during operation and transportation, and the survival rate is low. The test tube seedlings that have just grown roots are both resistant to storage and transportation, and easy to transplant. The survival line is high. The method is: cutting 3cm or more of the strong buds into the rooting medium, generally after 7-10 days, after the roots of the stem stems are taken out of the primordial root, they are taken into the nutrition cup, and after 5-6 days, the roots are self-generated. The root has root hair and good absorption function. The method has a high survival rate and shortens the production cycle, and is also suitable for long-distance transportation.
(4) Bottles or pots for bottle insertion or pot insertion, containing peat or humus soil and fine sand, inserting 20-30 rootless seedlings per bottle, inserting depth of about 0.3-1.2cm, adding half of Ms Mineral elements and I趴1.0-5.0mB/L or IAA rooting nutrient solution, cultured under certain temperature, humidity and light. After 20 days, new roots were grown, and after 30 days, the secondary roots were transplanted when they grew to 8-12 cm. More instrument information: Chlorophyll meter http://Instrument_more/yq_85_1.html